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» Visualization of cellulose synthase demonstrates functional association with microtubules.
Visualization of cellulose synthase demonstrates functional association with microtubules.
|Title||Visualization of cellulose synthase demonstrates functional association with microtubules. |
|Publication Type||Journal Article |
|Year of Publication||2006 |
|Authors||Paredez AR, Somerville CR, Ehrhardt DW |
|Journal||Science (New York, N.Y.) |
|Date Published||2006 Jun 9 |
|Keywords||arabidopsis, Arabidopsis Proteins, Benzamides, Biological Transport, Cell Membrane, Cellulose, Enzyme Inhibitors, Glucosyltransferases, Microfibrils, Microscopy, Confocal, Microtubules, Plants, Genetically Modified, Recombinant Fusion Proteins, Tubulin |
Expression of a functional yellow fluorescent protein fusion to cellulose synthase (CESA) in transgenic Arabidopsis plants allowed the process of cellulose deposition to be visualized in living cells. Spinning disk confocal microscopy revealed that CESA complexes in the plasma membrane moved at constant rates in linear tracks that were aligned and were coincident with cortical microtubules. Within each observed linear track, complex movement was bidirectional. Inhibition of microtubule polymerization changed the fine-scale distribution and pattern of moving CESA complexes in the membrane, indicating a relatively direct mechanism for guidance of cellulose deposition by the cytoskeleton.</p>
|Alternate Journal||Science |