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Prefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.

TitlePrefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.
Publication TypeJournal Article
Year of Publication2008
AuthorsGu Y, Deng Z, Paredez AR, DeBolt S, Wang Z-Y, Somerville C
JournalProceedings of the National Academy of Sciences of the United States of America
Date Published2008 Nov 18
Keywordsarabidopsis, Arabidopsis Proteins, Cell Division, Cloning, Molecular, Dinitrobenzenes, Electrophoresis, Gel, Two-Dimensional, Hypocotyl, Microtubules, Molecular Chaperones, Multiprotein Complexes, Mutant Proteins, Mutation, Reproductive Isolation, Plant Roots, Protein Folding, Proteomics, Seedling, Sulfanilamides, Tubulin

Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.</p>

Alternate JournalProc. Natl. Acad. Sci. U.S.A.