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Prefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.

Submitted by Alexander-Paredez on

TitlePrefoldin 6 is required for normal microtubule dynamics and organization in Arabidopsis.
Publication TypeJournal Article
Year of Publication2008
AuthorsGu Y, Deng Z, Paredez AR, DeBolt S, Wang Z-Y, Somerville C
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue46
Pagination18064-9
Date Published2008 Nov 18
ISSN1091-6490
Keywordsarabidopsis, Arabidopsis Proteins, Cell Division, Cloning, Molecular, Dinitrobenzenes, Electrophoresis, Gel, Two-Dimensional, Hypocotyl, Microtubules, Molecular Chaperones, Multiprotein Complexes, Mutant Proteins, Mutation, Reproductive Isolation, Plant Roots, Protein Folding, Proteomics, Seedling, Sulfanilamides, Tubulin
Abstract

<p>
Newly translated tubulin molecules undergo a series of complex interactions with nascent chain-binding chaperones, including prefoldin (PFD) and chaperonin-containing TCP-1 (CCT). By screening for oryzalin hypersensitivity, we identified several mutants of Arabidopsis that have lesions in PFD subunits. The pfd6-1 mutant exhibits a range of microtubule defects, including hypersensitivity to oryzalin, defects in cell division, cortical array organization, and microtubule dynamicity. Consistent with phenotypic analysis, proteomic analysis indicates several isoforms of tubulins were reduced in pfd6-1. These results support the concept that the function of microtubules is critically dependent on the absolute amount of tubulins.</p>
DOI10.1073/pnas.0808652105
Alternate JournalProc. Natl. Acad. Sci. U.S.A.