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Mesophyll-Driven Blade Expansion in Pisum sativum var. argenteum Leaves

TitleMesophyll-Driven Blade Expansion in Pisum sativum var. argenteum Leaves
Publication TypeJournal Article
Year of Publication2015
AuthorsStahlberg R, Cleland RE, Van Volkenburgh E
JournalAmerican Journal of Plant Sciences
Start Page753
Date Published04/2015
Type of Articleoriginal research
KeywordsBlade Expansion, Buckling, Epidermal Control, Epinasty, Leaf Expansion, Tissue Tension

The growth rate of any multilayered plant organ is limited by the cell layer with the least extensibility.
The dicot leaf blade has two epidermal layers covering the mesophyll layers, in which the
vascular network is embedded. There has been a lingering uncertainty about which layer limits
the rate of blade expansion in dicot leaves. The current study made use of leaf strips cut from the
argenteum variety of Pisum sativum L., in which the epidermal layers can easily be removed with
minimal damage. After this procedure, the mesophyll showed accelerated growth in short-term
and long-term experiments and light and darkness. Extension of both layers is strongly promoted
by acidic solutions. Isolated mesophyll layers expand in response to light. This effect depends on
turgor pressure, photosynthesis, and the plasma membrane H+-ATPase. The data allow concluding
that expanding leaf blades share with axial stem organs a similar arrangement of tissue tension:
an expanding core tissue pushing against a restrictive epidermal envelope. In complete leaves,
partial removal of the epidermis from only one side of the blade causes a strong epinastic or hyponastic
response. Removal of matching epidermis strips from both sides of complete blades
causes the exposed mesophyll strip to elongate in excess of the neighboring tissue: it buckles.

Refereed DesignationRefereed