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FKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis

TitleFKF1 F-box protein mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis
Publication TypeJournal Article
Year of Publication2005
AuthorsImaizumi T, Schultz TF, Harmon FG, Ho LA, Kay SA
Date Published2005
ISBN Number1095-9203 (Electronic)0036-8075 (Linking)
Keywords*Gene Expression Regulation, Plant, Gene Expression Profiling, Amino Acid Sequence, Arabidopsis Proteins/biosynthesis/chemistry/*genetics/*metabolism, Arabidopsis/genetics/growth & development/*metabolism, Cell Nucleus/metabolism, DNA-Binding Proteins/biosynthesis/*genetics, Flowers/growth & development, Molecular Sequence Data, Mutation, Reproductive Isolation, Plants, Genetically Modified, Proteasome Endopeptidase Complex/metabolism, Recombinant Fusion Proteins/metabolism, Repressor Proteins/chemistry/genetics/*metabolism, RNA Interference, Transcription Factors/biosynthesis/*genetics, Transcription, Genetic

<p>The temporal control of CONSTANS (CO) expression and activity is a key mechanism in photoperiodic flowering in Arabidopsis. FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1) protein regulates CO transcription, although the molecular mechanism is unknown. We demonstrate here that FKF1 controls the stability of a Dof transcription factor, CYCLING DOF FACTOR 1 (CDF1). FKF1 physically interacts with CDF1, and CDF1 protein is more stable in fkf1 mutants. Plants with elevated levels of CDF1 flower late and have reduced expression of CO. CDF1 and CO are expressed in the same tissues, and CDF1 binds to the CO promoter. Thus, FKF1 controls daily CO expression in part by degrading CDF1, a repressor of CO transcription.</p>