Neutrophils are the most abundant circulating leukocytes in humans, comprising the first line of innate immune defense. As neutrophils migrate towards sites of infection and inflammation they encounter a highly heterogeneous environment. Tasked to navigate through microscale obstacles, neutrophils continuously engage in a decision-making process. I aspire to understand the cytoskeleton dynamics of neutrophils when facing a directional dilemma and the mechanical forces acting on their multi-lobed nuclei in health and disease.