The precision of localization-based super-resolution microscopy techniques fundamentally relies on the point-spread function of the optical system and the number of photons one can collect. Here, we report that by using a high-numerical aperture objective lens and a custom cryogenic stage, we are able to increase photon yield by 2-3 fold over room temperature, thereby achieving more precise super-resolution reconstructions of complex subcellular structures.
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High numerical aperture cryogenic localization microscopy: a technique for increased precision of super-resolution reconstructions
Dr. Marc NahmaniInstitution:
University of Washington TacomaSeminar date:
Monday, April 24, 2017 - 12:00Location:
Fields of interest: