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Epigenetic regulation of centromeres in early development: Fluorescence based technologies for long term, high spatio- temporal resolution

Paul Maddox
University of North Carolina at Chapel Hill | Assistant Professor and William Burwell Harrison Fellow, Department of Biology
Seminar date:
Monday, May 21, 2018 - 12:00
HCK 132
Centromeres are fundamental to eukaryotic cell division, required for establishing kinetochores tofacilitate faithful microtubule based chromosome segregation. The histone-H3 variant CENtromere-Protein-A (CENP-A) is thought to provide centromere identity and as such must be retained through each cell cycle. DNA replication halves the number of CENP-A containing nucleosomes at centromeres and work in various model systems has shown that new molecules are incorporated outside of S-phaseby a unique mechanism. Developing embryos proceed rapidly through cell cycles and the complex cellular structure make quantitative imaging techniques required for measuring CENP-A localizationdynamics challenging. We have devised several new imaging and analysis techniques to show that CENP-A levels decrease each cell cycle in early C. elegans development through incomplete replenishing in G2 of each cell cycle. These results are the first to quantify centromere epigenetic regulation form zygote to differentiated cells and highlight that CENP-A levels can vary normally in development.
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