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The twin spot generator for differential Drosophila lineage analysis.

Submitted by Anne E.-Sustar on

TitleThe twin spot generator for differential Drosophila lineage analysis.
Publication TypeJournal Article
Year of Publication2009
AuthorsGriffin R, Sustar A, Bonvin M, Binari R, del Valle Rodriguez A, Hohl AM, Bateman JR, Villalta C, Heffern E, Grunwald D, Bakal C
JournalNature methods
Volume6
Issue8
Pagination600-2
Date Published2009 Aug
ISSN1548-7105
KeywordsAnimals, Mushroom Bodies, Clone Cells, Drosophila melanogaster, Fluorometry, predation, Mitosis, Mutation, Recombination, Genetic
Abstract

<p>
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.</p>
Alternate JournalNat. Methods