Submitted by Gerold-Schubiger on
| Title | The twin spot generator for differential Drosophila lineage analysis |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Griffin R, Sustar A, Bonvin M, Binari R, del Valle Rodriguez A, Hohl AM, Bateman JR, Villalta C, Heffern E, Grunwald D, Bakal C |
| Journal | Nat Methods |
| Volume | 6 |
| Issue | 8 |
| Pagination | 600-2 %8 Aug %! The twin spot generator for differential Drosophila lineage analysis. %@ 1548-7105 |
| Date Published | 2009 |
| Keywords | Animals, Mushroom Bodies, Clone Cells, Drosophila melanogaster, Fluorometry, predation, Mitosis, Mutation, Recombination, Genetic |
| Abstract | In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dop... %+ Commissariat ‡ l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu %G eng |